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Audrius Bubinas Gražina Giedraitytė Lilija Kalėdienė Ona Nivinskiene Rita Butkiene 《Central European Journal of Biology》2008,3(1):61-68
A number of thermophilic bacteria capable of utilizing naphthalene as a sole source of carbon were isolated from a high-temperature
oilfield in Lithuania. These isolates were able to utilize several other aromatic compounds, such as anthracene, benzene,
phenol, benzene-1, 3-diol, protocatechuic acid as well. Thermophilic isolate G27 ascribed to Geobacillus genus was found to have a high aromatic compound degrading capacity. Spectrophotometric determination of enzyme activities
in cell-free extracts revealed that the last aromatic ring fission enzyme in naphthalene biotransformation by Geobacillus sp. G27 was inducible via protocatechuate 3, 4-dioxygenase; no protocatechuate 4, 5-dioxygenase, protocatechuate 2, 3-dioxygenase activities were detected.
Intermediates such as o-phthalic and protocatechuic acids detected in culture supernatant confirmed that the metabolism of
naphthalene by Geobacillus sp. G27 can proceed through protocatechuic acid via ortho-cleavage pathway and thus differs from the pathways known for mesophilic bacteria. 相似文献
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J. Bogdanowicz B. Pawłowska A. Ilnicka S. Gawlik-Zawiślak A. Jóźwiak B. Sobiczewska E. Zdzienicka L. Korniszewski J. Zaremba 《Journal of applied genetics》2010,51(2):215-217
Fluorescent in situ hybridization (FISH) was performed in 76 patients referred to our department because of intellectual disability
and dysmorphic features that can be related to subtelomeric microaberrations. In all the patients, conventional cytogenetic
methods revealed normal karyotype. Four (5.3%) subtelomeric rearrangements were detected by FISH: 2 subtelomeric 1p36 deletions,
an unbalanced translocation involving chromosomes 1 and 12 with 1p36 deletion, and a de novo balanced translocation involving
chromosomes 19 and 22. Thus, 3 cases of 1p36 subtelomeric deletion were found (3.95%). To confirm subtelomeric rearrangements
in 2 patients, comparative genomic hybridization (CGH) was applied. Moreover, 3 cases of polymorphism without phenotypic effects
were found: in 2 patients, the polymorphism involved the long arm of chromosome 2 (maternal derivative in both patients),
while in the third patient, a polymorphism of the long arm of chromosome 7 was diagnosed. The latter polymorphism was also
found in the patient’s mother and grandfather. 相似文献
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